Assessment of Ploy Dopamine Coated Fe3o4 Nanoparticles for Melanoma (B16-F10 and A-375) Cells Detection Publisher Pubmed

Summary: Polydopamine-coated Fe3O4 nanoparticles (Fe3O4@PDA) exhibit lower cytotoxicity than uncoated NPs and can serve as effective MR imaging contrast agents for melanoma cells. #Nanomedicine #MRImaging #CancerResearch

Beigi FH¹ ; Fatahian S² ; Shahbazigahrouei S³ ; Shahbazigahrouei D⁴ ; Farzadniya A⁵
Source: Anti-Cancer Agents in Medicinal Chemistry Published:2020

Abstract

Objective: Polydopamine coated iron oxide nanoparticles (Fe3O4@PDA NPs) were synthesized, characterized, and their MR imaging contrast agents and photothermal potency were evaluated on melanoma (B16-F10 and A-375) cells and normal skin cells. To this end, MTT assay, Fe concentration, and MR imaging of both coated and uncoated NPs were assessed in C57BL/6 mice. Methods: Fe3O4 nanoparticles were synthesized using co-precipitation, and coated with polydopamine. The cytotoxicity of Fe3O4 and Fe3O4@PDA NPs on melanoma cells, with different concentrations, were obtained using MTT assay. MR images and Fe concentrations of nanoprobe and nanoparticles were evaluated under in vivo conditions. Results: Findings indicated that uncoated Fe3O4 showed the highest toxicity in animal (B16-F10) cells at 450µg/ml after 72h, while the highest toxicity in human (A-375) cells were observed at 350µg/ml. These nanoparticles did not reveal any cytotoxicity to normal skin cells, despite having some toxicity features in A-375 cells. MR image signals in the tumor were low compared with other tissues. The iron concentration in the tumor was higher than that of other organs. Conclusion: It is concluded that the cytotoxicity of Fe3O4@PDA was found to be significantly lower than un-coated nanoparticles (p <0.001), which allows some positive effects on reducing toxicity. The prepared nano-probe may be used as a contrast agent in MR imaging. © 2020 Bentham Science Publishers.