Isolation of Shiga Toxin-Producing Escherichia Coli O157:H7/ Nm From Hamburger and Chicken Nugget Publisher



Miri A¹ ; Rahimi E¹ ; Mirlohi M¹ ; Mahaki B² ; Ghasemian Safaei H³
Source: International Journal of Environmental Health Engineering Published:2014

Abstract

Aims: This study was conducted to determine the prevalence rate, virulence genes and antimicrobial resistance of Escherichia coli O157:H7/NM isolated from hamburger and chicken nugget in Isfahan, Iran. Material and Methods: From June 2013 to July 2013, a total of 190 hamburger (120) and chicken nugget (70), were collected from four randomly selected factories in Isfahan, Iran. They were evaluated for the presence of E. coli O157:H7/NM using microbiological culture and polymerase chain reaction. Statistical analysis was performed using SPSS software version 16.0 (SPSS Inc., Chicago o, IL, USA). Results: From a total of 190 samples analyzed four samples (2.1%) were contaminated with E. coli O157. All of the E. coli O157 were isolated from hamburger samples (3.3%) and chicken nugget samples were negative. Of four E. coli O157 isolated, only one sample was serotype E. coli O157:H7 and others were serotype E. coli O157:NM. Among four E. coli O157:H7/NM isolates, one strain was positive for all stx1, stx2, eaeA and ehxA genes. One strain was positive for stx2 gene. The other two were negative for these genes. All isolates (100%) were resistant to one or more antimicrobial agents. Conclusions: The results of this study showed that hamburger could be a significant source of E. coli O157:H7 and E. coli O157:NM serotypes in Iran and multi-resistance was found in 27% of E. coli O157 strains and this is a major public health concern. © 2014 Miri A. This is an open‑access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.