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Antigenicity and Immunogenicity of Fused B-Subunit of Heat Labile Toxin of Escherichia Coli and Colonization Factor Antigen I Polyepitopes Publisher Pubmed



Savar NS1 ; Dashti A1 ; Darzi Eslam E1 ; Jahaniannajafabadi A2 ; Jafari A1
Authors

Source: Journal of Microbiological Methods Published:2014


Abstract

Linear B-cell epitopes (93AKEFEAAAL101 and 66PQLTDVLN73) of CfaB were genetically fused to ltb-(gly)5-cfaB(1-25). Sera of rabbits immunized with fusion proteins reacted strongly with solid-phase bound ETEC bacteria bearing CFA/I fimbriae. Sera failed to agglutinate or inhibit hemagglutination promoted by CFA/I-positive strain which may be due to solvent inaccessibility of epitope residues on intact fimbriae. © 2014 Elsevier B.V.