Induction of Neuron-Like Cells From Adipose Derived Stem Cells in Alginate Hydrogel, Using Neurospheres Formation



Razavi S¹ ; Khosravizadeh Z² ; Bahramian H¹ ; Kazemi M³
Source: Journal of Isfahan Medical School Published:2014

Abstract

Background: Hydrogels provide appropriate three-dimensional environment for culture of a variety of cells and cell encapsulation in hydrogels is a promise plan for tissue engineering applications. Alginate is an attractive biocompatible hydrogel that provides a supportive system for the encapsulated cells. Moreover, human adipose derived stem cells are mesenchymal stem cells that might be a suitable source of cells for use in autologous cell therapy. Methods: In this study, we examined the fate of human adipose derived stem cells encapsulated in alginate hydrogel that cultured in neural induction medium for 1 week. Using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay and immunocytochemical analysis, the proliferation rate, and viability and neural differentiation of human adipose derived stem cells were evaluated. Findings: We observed a significant increase in the mean percent of Nestin, glial fibrillary acidic protein (GFAP) and microtubule-associated protein-2 (MAP2) positive cells and significant reduction of proliferation rate and viability in encapsulated cells versus monolayer induced cells. Conclusion: These findings showed that alginate hydrogel can provide a suitable environment for neural differentiation of human adipose derived stem cells. © 2014, Isfahan University of Medical Sciences(IUMS). All rights reserved.