Regeneration of Rat Sciatic Nerve Using Plga Conduit Containing Rat Adscs With Controlled Release of Bdnf and Gold Nanoparticles Publisher Pubmed

Summary: Research shows gold nanoparticles and stem cells in scaffolds boost nerve repair, promising better recovery. #NerveRepair #Biomaterials

Jahromi M¹ ; Razavi S¹ ; Seyedebrahimi R¹ ; Reisi P² ; Kazemi M³
Source: Journal of Molecular Neuroscience Published:2021

Abstract

Implantation of a nerve guidance conduit (NGC) carrying neuroprotective factors is promising for repairing peripheral nerve injury. Here, we developed a novel strategy for repairing peripheral nerve injury by gold nanoparticles (AuNPs) and brain-derived neurotrophic factor (BDNF)–encapsulated chitosan in laminin-coated nanofiber of Poly(l-lactide-co-glycolide) (PLGA) conduit and transplantation of rat adipose–derived stem cells (r-ADSCs) suspended in alginate. Then, the beneficial effect of AuNPs, BDNF, and r-ADSCs on nerve regeneration was evaluated in rat sciatic nerve transection model. In vivo experiments showed that the combination of AuNPs- and BDNF-encapsulated chitosan nanoparticles in laminin-coated nanofiber of PLGA conduit with r-ADSCs could synergistically facilitate nerve regeneration. Furthermore, the in vivo histology, immunohistochemistry, and behavioral results demonstrated that the AuNPs- and BDNF-encapsulated chitosan nanoparticles in NGC could significantly reinforce the repair performance of r-ADSCs, which may also contribute to the therapeutic outcome of the AuNPs, BDNF, and r-ADSCs strategies. In this study, we found that the combination of AuNPs and BDNF releases in NGC with r-ADSCs may represent a new potential strategy for peripheral nerve regeneration. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.