A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (Cop-Gfp) in Baculovirus Expression System Pubmed



Shokrollahi N¹ ; Shahbazzadeh D¹ ; Pooshangbagheri K¹ ; Habibianbouhi M² ; Jahaniannajafabadi A³ ; Behdani M¹
Source: Iranian Biomedical Journal Published:2016

Abstract

Background: Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells. Methods: The aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluorescent protein (copGFP). In this light, the resultant vector was constructed and used for transfection of Spodoptera frugiperda cells. Results: Expression of the copGFP protein in insect cells was confirmed by fluorescent microscopy and Western-blot analysis. Conclusion: The application of copGFP control bacmid can be considered as an appropriate control for insect cell transfection. © 2016, Pasteur Institute of Iran. All rights reserved.