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The Effect of Purmorphamine on Differentiation of Endometrial Stem Cells Into Osteoblast-Like Cells on Collagen/Hydroxyapatite Scaffolds Publisher Pubmed



Bahrami N1, 2, 3 ; Malekolkottab F4 ; Ebrahimibarough S5 ; Alizadeh Tabari Z4 ; Hamisi J4 ; Kamyab A6 ; Mohamadnia A7, 8 ; Ai A1 ; Bayat F1 ; Bahrami N1, 2, 3 ; Ai J5
Authors

Source: Artificial Cells# Nanomedicine and Biotechnology Published:2017


Abstract

We assessed the effect of purmorphamine along with collagen/hydroxyapatite scaffold in inducing osteogenesis of human endometrial stem cells (hEnSCs). The adhesion, viability, proliferation, and differentiation of cells on scaffold were assayed with SEM, MTT, real time-PCR, and ALP assay, respectively. The results were shown good integration of cells with scaffold. Also, qRT-PCR of differentiated cells shows that osteoblast cell markers are expressed after 21d in 2D and scaffold groups while in the scaffold group the expression of these markers were higher than the 2D group. Based on our findings, collagen/hydroxyapatite scaffold with PMA has the potential role in osteogenic differentiation of hEnSCs. © 2016 Informa UK Limited, trading as Taylor & Francis Group.
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