Introducing Critical Genes Related to X-Ray Radiation in Human Bronchial Epithelial Beas-2B Cells



Rezaei M ; Razi F ; Ahmadzadeh A ; Bandarian F ; Arjmand B ; Rezaeitavirani M
Source: Tanaffos Published:2025

Abstract

Background: X-ray radiation application in radiology is a challenging subject for patients. Understanding the molecular events triggered by X-ray radiation can provide a suitable protocol for maintaining patient health. The primary aim of this study is to explore crucial affected genes in this regard. Materials and Methods: Extraction and validation of data from the Gene Expression Omnibus (GEO) database is the first step in identifying significantly differentially expressed genes (DEGs). Analysis of gene expression profiles of human bronchial epithelial BEAS-2B cells through a directed protein-protein interaction (PPI) network was used to identify the key targeted genes affected by X-ray radiation. Results: Results of the analyzed directed PPI networks, based on expression, activation, inhibition, and reaction actions, and considering outdegree values, showed that IL1B, CCND1, RAC2, NDC80, and MIB1 are the crucial genes targeted by X-ray. Conclusion: In conclusion, upregulation of IL1B, RAC2, and CCND1, which are associated with cellular damages and downregulation of NDC80 and MIB1, which protect the treated cells, were pointed out as the major molecular events in response to X-ray radiation. © 2025 NRITLD, National Research Institute of Tuberculosis and Lung Disease, Iran.