Spectrophotometric Determination of Trace Co (Ii) With Naphthylglyoxalaldoxime As a Newly Synthesized Reagent



Tehrani MB¹ ; Jalaeian B¹ ; Shamsa H¹ ; Souri E¹
Source: Research Journal of Pharmaceutical# Biological and Chemical Sciences Published:2016

Abstract

A simple, sensitive and accurate spectrophotometric method for the determination of Co(II) using newly synthesized reagent, 2-(naphthalen-2-yl)-2-oxoacetaldehyde oxime, has been developed. The reagent, 2-(naphthalen-2-yl)-2-oxoacetaldehyde oxime (2NGA), was synthesized based on the reaction of 2-acethyl naphthalene with amyl nitrite in sodium methoxide and reflux at 40°C for 50h. 2NGA reacts with Co2+ to form stable yellow complex in basic media which is easily extractable with chloroform. Beer's law was obeyed over the concentration range of 1-15 μg/ml with r2 = 0.9990 and RSD of slope 0.822. Within-day and between-day precision and accuracy values were less than 2.6%. Finally, the method has been applied to a vit B12 product successfully and the results compared with atomic absorption method. The results showed that there was no significant difference between two methods.