Assessment of the Impact of Diflubenzuron on the Inhibition of Adult Emergence in Aedes Aegypti (Diptera: Culicidae) From Hormozgan Province, Iran, 2025 Publisher



Ebrahimi S ; Vatandoost H ; Oshaghi MA ; Azizi K ; Khanavi M ; Moosakazemi SH
Source: Journal of Arthropod-Borne Diseases Published:2025

Abstract

Background: The presence of Aedes aegypti in Iran and the risk of transmitting viral diseases, such as dengue and Zika, underscore the importance of evaluating effective vector control methods. Methods: This study investigated the effectiveness of diflubenzuron, an insect growth regulator that inhibits chitin synthesis, in preventing adult emergence of Ae. aegypti larvae from Hormozgan. The concentrations of 0.03, 0.06, 0.12 and 0.24 mg/L of 25% wettable powder formulations of diflubenzuron were used, according to WHO protocols. Results: Diflubenzuron was highly effective at 0.24 mg/L, achieving 98% emergence inhibition (EI50=0.053 mg/L, EI90=0.146 mg/L). At lower concentrations, e.g., 0.12 mg/L, emergence inhibition decreased to 83%, indicating a clear concentration-dependent reduction in efficacy. Larvae exposed to sublethal doses displayed abnormal movements and reduced responses to stimuli. Conclusion: This experiment supports diflubenzuron’s potential as an effective larvicide for vector control programs in Iran. However, field trials and resistance monitoring are recommended before widespread implementation. Copyright © 2025 The Authors.