Improvement of Cytotoxicity and Necrosis Activity of Ganoderic Acid a Through the Development of Pmbn-A.Her2-Ga As a Targeted Nano System Publisher



Motamed Fath P¹ ; Rahimnejad M^{2, 3} ; Moradikalbolandi S⁴ ; Ebrahimi Hosseinzadeh B¹ ; Jamshidnejadtosaramandani T⁵
Source: RSC Advances Published:2022

Abstract

The targeting nano carriers have been promptly proposed to overcome the current obstacles in conventional chemotherapy approaches for cancer. Currently, PMBN (poly[MPC-co-(BMA)-co-(MEONP)]), is considered as a promising amphiphilic polymer that could be easily targeted and conjugated to hydrophobic substances with low bioavailability. To target breast cancer cells overexpressing human epidermal receptor 2 (HER2) receptors, anti-HER2 monoclonal antibody (A.Her2) was conjugated to PMBN and afterward loaded with ganoderic acid A (GA-A) as an anti-cancer metabolite. The efficacy of conjugation and loading was reasonably favourable. The rod shape of the polymer with a size of approximately 160 ± 30 nm was confirmed. Our results indicated that PMBN-A.Her2-GA is an anionic nanostructure with an appropriate form and capable of being applied in cancer therapy. Subsequently, cytotoxicity analysis revealed an improved anti-proliferative effect of GA-A. © The Royal Society of Chemistry.