A Silk Fibroin/Decellularized Extract of Wharton’S Jelly Hydrogel Intended for Cartilage Tissue Engineering

A Silk Fibroin/Decellularized Extract of Wharton’S Jelly Hydrogel Intended for Cartilage Tissue Engineering Publisher

Basiri A¹ ; Farokhi M² ; Azami M¹ ; Ebrahimibarough S¹ ; Mohamadnia A³ ; Rashtbar M¹ ; Hasanzadeh E¹ ; Mahmoodi N⁴ ; Baghaban Eslaminejad M⁵ ; Ai J¹

Source: Progress in Biomaterials Published:2019

Abstract

A hybrid hydrogel was obtained from decellularized extract from Wharton’s jelly (DEWJ) and silk fibroin (SF) and characterized for cartilage tissue engineering. Wharton’s jelly was used due to its similarity with articular cartilage in extracellular matrix composition. Also, silk fibroin has good mechanical properties which make this construct appropriate for cartilage repair. Decellularization of Wharton’s jelly was verified by DAPI staining, DNA quantification, and PCR analysis. Then, the biochemical composition of DEWJ was determined by ELISA kits for total proteins, collagens, sulfated glycosaminoglycans (sGAG), and transforming growth factor β1 (TGF-β1). After fabricating pure SF and SF/DEWJ hybrid hydrogels, their physical and mechanical properties were characterized by FESEM, Fourier-transform infrared spectroscopy (FTIR) and rheological assays (amplitude and frequency sweeps). Furthermore, cell viability and proliferation were assessed by MTT assay. The results have shown that DEWJ in hybrid hydrogels enhances mechanical properties of the construct relative to pure SF hydrogels. Also, this extract at its 40% concentration in culture media and 20% or 40% concentrations in SF/DEWJ hybrid hydrogels significantly increases population of the cells compared to control and pure SF hydrogel after 7 days. In conclusion, this study proposes the potential of SF/DEWJ hybrid hydrogels for cartilage tissue engineering applications. © 2019, The Author(s).

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Mahmoud Azami (3)

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Style	Citing Format
MLA	Basiri A, et al.. "A Silk Fibroin/Decellularized Extract of Wharton’S Jelly Hydrogel Intended for Cartilage Tissue Engineering." Progress in Biomaterials, vol. 8, no. 1, 2019, pp. 31-42.
APA	Basiri A, Farokhi M, Azami M, Ebrahimibarough S, Mohamadnia A, Rashtbar M, Hasanzadeh E, Mahmoodi N, Baghaban Eslaminejad M, Ai J (2019). A Silk Fibroin/Decellularized Extract of Wharton’S Jelly Hydrogel Intended for Cartilage Tissue Engineering. Progress in Biomaterials, 8(1), 31-42.
Chicago	Basiri A, Farokhi M, Azami M, Ebrahimibarough S, Mohamadnia A, Rashtbar M, Hasanzadeh E, Mahmoodi N, Baghaban Eslaminejad M, Ai J. "A Silk Fibroin/Decellularized Extract of Wharton’S Jelly Hydrogel Intended for Cartilage Tissue Engineering." Progress in Biomaterials 8, no. 1 (2019): 31-42.
Harvard	Basiri A et al. (2019) 'A Silk Fibroin/Decellularized Extract of Wharton’S Jelly Hydrogel Intended for Cartilage Tissue Engineering', Progress in Biomaterials, 8(1), pp. 31-42.
Vancouver	Basiri A, Farokhi M, Azami M, Ebrahimibarough S, Mohamadnia A, Rashtbar M, et al.. A Silk Fibroin/Decellularized Extract of Wharton’S Jelly Hydrogel Intended for Cartilage Tissue Engineering. Progress in Biomaterials. 2019;8(1):31-42.
BibTex	@article{ author = {Basiri A and Farokhi M and Azami M and Ebrahimibarough S and Mohamadnia A and Rashtbar M and Hasanzadeh E and Mahmoodi N and Baghaban Eslaminejad M and Ai J}, title = {A Silk Fibroin/Decellularized Extract of Wharton’S Jelly Hydrogel Intended for Cartilage Tissue Engineering}, journal = {Progress in Biomaterials}, volume = {8}, number = {1}, pages = {31-42}, year = {2019} }
RIS	TY - JOUR AU - Basiri A AU - Farokhi M AU - Azami M AU - Ebrahimibarough S AU - Mohamadnia A AU - Rashtbar M AU - Hasanzadeh E AU - Mahmoodi N AU - Baghaban Eslaminejad M AU - Ai J TI - A Silk Fibroin/Decellularized Extract of Wharton’S Jelly Hydrogel Intended for Cartilage Tissue Engineering JO - Progress in Biomaterials VL - 8 IS - 1 SP - 31 EP - 42 PY - 2019 ER -

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