Evaluation of Deltamethrin in Combination of Piperonyl Butoxide (Pbo) Against Pyrethroid Resistant, Malaria Vector, Anopheles Stephensi in Irs Implementation: An Experimental Semi-Filed Trial in Iran



Nikpour F¹ ; Vatandoost H^{1, 2} ; Hanafibojd AA^{1, 2} ; Raeisi A³ ; Ranjbar M⁴ ; Enayati AA⁵ ; Abai MR^{1, 2} ; Shayeghi M¹ ; Mojahedi AR⁶ ; Pourreza A¹
Source: Journal of Arthropod-Borne Diseases Published:2017

Abstract

Background: The aim of this study was to evaluate different concentrations of deltamethrin combined with formu-lated piperonyl butoxide (PBO) synergist on various surfaces against the wild strain of Anopheles stephensi, the main malaria vector in Southern Iran under semi-field condition. Methods: Four concentrations of deltamethrin WG 25% (Tagros) and PBO 800EC-UV (Endura) were prepared and sprayed on the pre-designed surfaces in accordance with WHO alliance line of the IRS Micronair®. The WHO's rec-ommended bioassay kit and method was used during this study. Results: Comparing the mortality rate of mosquitoes, the results showed a significant difference between months after treatment of IRS (Indoor Residual Spraying) (P < 0.05) but didn't show any significant differences between days during the first and second months (P > 0.05). Statistical test revealed a significance difference between mortality rate of mosquitoes in exposing to concentrations of 1 and 4 (P < 0.05) which demonstrated effect of synergizing PBO on mortality rate. Conclusion: This research as the first semi-field trial on deltamethrin added to different concentrations of formulat-ed PBO for IRS, indicates that deltamethrin+10X PBO is more effective than other concentrations. Therefore, using synergists can be suggested as a new tool for prevention of pyrethriod resistance, although more studies are recom-mended.