Association Between Mthfr Variant and Diabetic Neuropathy Publisher Pubmed



Kakavand Hamidi A¹ ; Radfar M^{2, 3} ; Amoli MM¹
Source: Pharmacological Reports Published:2018

Abstract

Background Methylene-tetrahydrofolate reductase (MTHFR) gene variant may play an important role in the pathophysiology of diabetes and its complications due to its influence on plasma homocysteine levels and also its effect on scavenging peroxynitrite radicals. Diabetic peripheral neuropathy (DPN) is one of the most common diabetic chronic complications. The aim of this study was to investigate the relationship between diabetic neuropathy and MTHFR gene C677T and 1298A ⁄C polymorphisms. Method Patients with type 2 diabetes N = 248 were enrolled in the study, consisting of patients with neuropathy (N = 141) and patients without neuropathy (N = 107). MTHFR C677T polymorphism was analyzed using polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP) of genomic DNA for genotyping of samples. 1298A/C polymorphism was evaluated using ARMS-PCR. Result There was a significant difference in MTHFR polymorphism between the groups with and without neuropathy. Conclusion Our results suggest that MTHFR 677 variant confer risk for diabetic neuropathy among Iranian patients with type 2 diabetes. © 2017 Institute of Pharmacology, Polish Academy of Sciences