Effects of Horsetail, Alfalfa, Ortie, Chene and Aleppo Oak As Potential Hemostatic Agents on Laboratory Coagulation Tests Publisher



Ahmadianfar S^{1, 2} ; Mehrabi N³ ; Mohammadi S^{4, 5} ; Sobhanizadeh A⁶ ; Moradabadi A⁷ ; Norooziaghideh A²
Source: Natural Product Sciences Published:2023

Abstract

This study investigated the effect of ethanol extracts of horsetail, alfalfa, ortie, chene and aleppo oak on blood coagulation in vitro. Extraction was performed by the maceration method. Extracts were mixed with platelet and plasma, then prothrombin time (PT), activated partial thromboplastin time (APTT) and platelet aggregation tests were conducted. Alfalfa extract had a dose-dependent effect on the PT. Ortie, and horsetail, reduced the PT significantly compared to control group. Alfalfa, horsetail, and ortie reduced the APTT, but their effect was insignificant compared to the control group. The pooled extract showed the highest effect compared to the single extracts in a dose-dependent manner. Horsetail and alfalfa induced platelet aggregation in response to arachidonic acid but not in response to collagen. In the case of ortie, no aggregation occurred regarding the arachidonic acid, and incomplete was observed in response to collagen. Interestingly, blood clotting occurred immediately after adding the chene, aleppo oak and the pooled extract, and therefore platelet poor plasma (PPP) and platelet rich plasma (PRP) became jelly. Generally, chene and aleppo oak, as well as pooled extract, were more effective in inducing both primary and secondary coagulation pathways via shortening the PT and APTT, and induction of platelet aggregation. © 2023, Korean Society of Pharmacognosy. All rights reserved.