Primordial Germ Cell Differentiation of Nuclear Transfer Embryonic Stem Cells Using Surface Modified Electroconductive Scaffolds Publisher Pubmed



Eslamiarshaghi T¹ ; Vakilian S¹ ; Seyedjafari E² ; Ardeshirylajimi A^{1, 3} ; Soleimani M⁴ ; Salehi M^{5, 6}
Source: In Vitro Cellular and Developmental Biology - Animal Published:2017

Abstract

A combination of nanotopographical cues and surface modification of collagen and fibronectin is a potential platform in primordial germ cells (PGCs) differentiation. In the present study, the synergistic effect of nanotopography and surface modification on differentiation of nuclear transfer embryonic stem cells (nt-ESCs) toward PGC lineage was investigated. In order to achieve this goal, poly-anyline (PANi) was mix within poly-l-lactic acid (PLLA). Afterward, the random composite mats were fabricated using PLLA and PANi mix solution. The nanofiber topography notably upregulated the expressions of prdm14, mvh and c-kit compared with tissue culture polystyrene (TCP). Moreover, the combination of nanofiber topography and surface modification resulted in more enhancement of PGCs differentiation compared with non-modified nanofibrous scaffold. Additionally, gene expression results showed that mvh and c-kit were expressed at higher intensity in cells exposed to collagen and fibronectin rather than collagen or fibronectin solitary. These results demonstrated the importance of combined effect of collagen and fibronectin in order to develop a functional extracellular matrix (ECM) mimic in directing stem cell fate and the potential of such biofunctional scaffolds for treatment of infertility. © 2016, The Society for In Vitro Biology.