Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share By
Evaluation of Lncrna Sox2ot Gene Expression in Breast Cancer Patients and Its Association With Prognosis of Disease: A Paired Sample Study Publisher



M Yousefi MAHSHID ; M Karimi MEHDI ; A Rezaei ABOLHASAN ; M Mortezazadeh MASOUD ; S Akhshabi SHEYDA ; A Yousefi Ladmakhi ALIREZA ; N Shirsalimi NIYOUSHA ; E Kamali Yazdi EHSAN ; St Seyyed Mahmoudi Seyyed TAHER ; M Kashani MEHDI
Authors

Source: Egyptian Journal of Medical Human Genetics Published:2025


Abstract

Background and aim: Breast cancer is a complex disease with genetic and molecular alterations. Long non-coding RNAs (lncRNAs), particularly SOX2 overlapping transcript (SOX2OT), are key regulatory molecules in cancer biology. Understanding SOX2OT expression patterns could provide insights into its potential as a biomarker for diagnosis and a target for therapeutic intervention. This study aimed to evaluate SOX2OT gene expression in breast cancer patients and its association with the disease’s prognosis. Methods: This study was conducted in Tehran, Iran, in 2020. A total of 40 breast tissue samples were collected from 20 breast cancer patients, including 20 samples from tumor tissues and 20 from adjacent non-tumor marginal areas of the same patients. RNA extraction, cDNA synthesis, and Real-Time PCR analysis were performed, and the results were evaluated using SPSS statistical software. Results: The results from RT-PCR showed that SOX2OT gene expression in cancer tumor samples was significantly 3.8 times higher than in non-tumor (control) samples (Fold change = 3.8; p = 0.025). This elevation was notably higher in patients with advanced-stage (III & IV) breast cancer (p = 0.02). A significant correlation was observed between SOX2OT expression and a family history of breast cancer (p = 0.031). In contrast, non-significant associations were found with other factors such as tumor size, lymphatic and vascular invasion, tissue necrosis, or smoking history. Conclusion: The SOX2OT gene expression is significantly elevated in breast cancer tumors, particularly in advanced stages, suggesting its potential involvement in disease progression. The significant correlation between SOX2OT expression and a family history of breast cancer highlights a possible genetic influence. However, the lack of association with other clinical factors such as tumor size, tissue invasions, or smoking history suggests that SOX2OT expression may be more specifically linked to genetic predisposition and disease advancement rather than these factors. © 2025 Elsevier B.V., All rights reserved.
Related Docs
View other Related Docs
1. Long Non-Coding Rnas Expression in Breast Cancer: Cbr3-As1 Lncrna As a Sensitive Biomarker, Asian Pacific Journal of Cancer Prevention (2021)
2. Distinct Gene Expression Patterns of Sox2 and Sox2ot Variants in Different Types of Brain Tumours, Journal of Genetics (2023)
3. Down-Regulation of Meg3, Panda and Casc2 As P53-Related Lncrnas in Breast Cancer, Breast Disease (2022)
Experts (# of related papers)
View all Related Experts
Lobat Geranpayeh (2)
Alireza Ghanadan (1)
Other Related Docs
4. Prognostic and Diagnostic Values of Non-Coding Rnas As Biomarkers for Breast Cancer: An Umbrella Review and Pan-Cancer Analysis, Frontiers in Molecular Biosciences (2023)
5. Expression and Clinicopathological Significances of Lncrnas: Could Ara and Zeb2nat Be the Potential Breast Cancer-Related Biomarkers?, Archives of Medical Research (2020)
6. Expression Profiling Revealed Up-Regulation of Three Lncrnas in Breast Cancer Samples., Experimental and Molecular Pathology (2020)
7. Expression Analysis of Four Long Noncoding Rnas in Breast Cancer, Tumor Biology (2016)
8. Expression Signature of Lncrna Aptr in Clinicopathology of Breast Cancer: Its Potential Oncogenic Function in Dysregulation of Erbb Signaling Pathway, Gene Reports (2021)
9. Expression Levels of Breast Cancer-Related Gas5 and Lsinct5 Lncrnas in Cancer-Free Breast Tissue: Molecular Associations With Age at Menarche and Obesity, Breast Journal (2018)
10. Evaluation of the Expression Level of Some Coding and Non-Coding Genes in Breast Cancer Samples Based on Bioinformatics and Laboratory Studies, International Journal of Cancer Management (2024)
11. A Novel Natural Antisense Transcript at Human Sox9 Locus Is Down-Regulated in Cancer and Stem Cells, Biotechnology Letters (2020)
12. Assessment of Expression of Lncrna H19, Pinin and Their Relation to Rs532 Single Nucleotide Polymorphism in the Pinin Gene Present in the Tissue of Individuals Suffering From Breast Cancer in Central Iran, Current Cancer Therapy Reviews (2022)
13. Evaluation of Beclin1 and Mtor Genes and P62 Protein Expression in Breast Tumor Tissues of Iranian Patients, Molecular Biology Research Communications (2024)
14. Breast Cancer-Linked Lncrna U-Eleanor Is Upregulated in Breast of Healthy Women With Lack or Short Duration of Breastfeeding, Journal of Cellular Biochemistry (2019)
15. Investigating Socioeconomic Inequality in the Incidence of Breast Cancer in Iran: Interprovincial Analysis and Comparison, Scientific Journal of Kurdistan University of Medical Sciences (2025)
16. Comparison of Sox10 Gene Expression in Melanoma and Melanocytic Nevus Samples Using Real-Time Pcr and Immunohistochemistry, Gene Reports (2020)
17. Refining Breast Cancer Biomarker Discovery and Drug Targeting Through an Advanced Data-Driven Approach, BMC Bioinformatics (2024)
18. Up-Regulation of Foxn3-As1 in Invasive Ductal Carcinoma of Breast Cancer Patients, Heliyon (2021)
19. Decreased Sox2 Messenger Rna Expression in Basal Cell Carcinoma, Indian Journal of Dermatology (2020)
20. Expression of Mirnas Targeting Mtor and S6k1 Genes of Mtor Signaling Pathway Including Mir-96, Mir-557, and Mir-3182 in Triple-Negative Breast Cancer, Applied Biochemistry and Biotechnology (2018)