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Sequential Monitoring of Tim-3 Mrna Expression in Blood and Urine Samples of Renal Transplant Recipients Publisher Pubmed



Shahbaz SK1, 2 ; Barabadi M1 ; Ahmadpour P3 ; Pourrezagholi F3 ; Nafar M3 ; Foroughi F4 ; Hosseinzadeh M5 ; Ghorbanpour M6 ; Yekaninejad MS6 ; Amirzargar A1, 7
Authors

Source: Transplant Immunology Published:2019


Abstract

Background: T cell immunoglobulin and mucin domain 3 (TIM-3), as a co-inhibitory receptor expressed on Th1, Th17, CD8T, FoxP3 + Treg and innate immune cells, plays an important role in suppression of T cell-mediated immune responses, tolerance induction and T cell exhaustion. In this study, we evaluated sequential alterations of TIM-3 mRNA expression level in blood and urine samples of renal transplant recipients to predict approaching clinical episodes. Methods: A total of 52 adult renal transplant recipients (31 male and 21 female)were enrolled in this study. All the patients received kidney transplant from living unrelated donors. TIM-3 mRNA expression in peripheral blood mononuclear cells (PBMCs)and urinary cells were quantified using Real Time TaqMan polymerase chain reaction (PCR)at 4 different time points (pre-transplantation, 2, 90 and 180 days post-transplantation). Result: TIM-3 mRNA expression level on days 2, 90 and 180 after transplantation was significantly higher in blood and urine samples of patients with graft dysfunction (GD)compared with patients with well-functioning graft (WFG). Our results also showed a high correlation between blood and urinary level of TIM-3 mRNA expression. The data from Receiver Operating Characteristic (ROC)Curve Analysis showed that blood and urinary TIM-3 mRNA expression level at month 3 and 6 could discriminate graft dysfunction (GD)from well-functioning graft (WFG)with high specificity and sensitivity. Conclusion: Our data suggested that serial monitoring of TIM-3 mRNA level in the blood and urine samples of renal transplant recipients could be a useful non-invasive biomarker for prediction and diagnosis of allograft dysfunction. © 2018 Elsevier B.V.
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