A Novel Dna/Hemin Complex With Enzyme-Like Activity Selected From a Hairpin Dnas Library at Zero H2o2 Concentration Publisher



Shokri E¹ ; Hosseini M² ; Boldaji MN² ; Shahsavar K² ; Amirisadeghan A³ ; Nasiri N⁴ ; Bahmani A² ; Ganjali MR^{5, 6} ; Saboury AA⁷
Source: Molecular Catalysis Published:2022

Abstract

Here, a library of short DNAs possessing stem-loop conformation is examined for enzyme-like activity at zero H2O2 concentration. The stem part of all hairpins was identical and the loop sequences were diversified with runs of different nucleotides (A, T, C, G). According to the results, we found that G-type hairpins with 3, 4 and 5 guanines in the loop part are able to be complex with hemin and, oxidize TMB. Among them, a complex named G5HH showed an apparent oxidase-like activity and, was chosen for further studies. UV-thermal analysis, gel shift assay, mutation and structural tests with molecular docking results confirmed that G-tetrads are not formed in our experimental system. So, in this study we describe the functionality of a distinct type of DNA in the hairpin fold as a new class of DNAzymes. Discovering such property for simple DNA hairpins would hold great promise for wide applications in bio-nanotechnology and other fields of research. © 2022 Elsevier B.V.