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Gene Expression and Levels of Il-6 and Tnfα in Pbmcs Correlate With Severity and Functional Class in Patients With Chronic Heart Failure Publisher Pubmed



Eskandari V1 ; Amirzargar AA1 ; Mahmoudi MJ2 ; Rahnemoon Z3 ; Rahmani F4, 5 ; Sadati S1 ; Rahmati Z1 ; Gorzin F1 ; Hedayat M6, 7 ; Rezaei N1, 4, 5
Authors

Source: Irish Journal of Medical Science Published:2018


Abstract

Background: Evidence shows that proinflammatory cytokines are important determinants of assessment of severity and prognosis of chronic heart failure (CHF). Aims: We investigated whether peripheral expression of the proinflammmatory factors, TNF-α and IL-6 can predict variable of clinical assessment of patients with CHF. Methods: In this report, we used real-time PCR assay to compare relative gene expression of TNFα and IL-6 in PBMC from CHF patients with various heart diseases (n = 42, EF < 45%, NYHA I to IV) and matched healthy control subjects (n = 42).We also determined the TNFα and IL-6 concentrations of cell culture supernatant of PBMCs with ELISA. Results: There was a significant negative correlation between gene expression of TNFα and LVEF(r = 0.4, p < 0.05). Patients with CHF had increased gene expression of TNFα and IL-6 in PBMCs (p < 0.05). They also had elevated the supernatant levels of these cytokines in cultured PBMCs (p < 0.001). Levels of TNFα and IL-6 were increased in ischemic heart disease compared to non-ischemic heart disease. There was a positive correlation between TNFα and IL-6 levels in CHF patients and severity of CHF in patients. Levels of these cytokines were higher in patients with NYHA III–IV than in NYHA I–II and normal subjects. Conclusions: Results of this study indicate that peripheral expression of proinflammatory cytokines, TNF-α and IL-6, is important indicators of severity and prognosis in patients with chronic heart disease. © 2017, Royal Academy of Medicine in Ireland.
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