Gas Chromatography–Mass Spectrometry Determination of Pregabalin in Human Plasma Using Derivatization Method Publisher



Tafesse TB^{1, 2, 3} ; Mazdeh FZ⁴ ; Chalipour A⁴ ; Tavakoli M⁴ ; Hajimahmoodi M^{4, 5} ; Amini M²
Source: Chromatographia Published:2018

Abstract

A gas chromatography–mass spectrometry method for the determination of pregabalin in human plasma is described. The procedure involves precipitation of protein, liquid–liquid extraction with ethylene glycol monomethyl ether, and derivatization with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide in the presence of N-hydroxysuccinimide as additive. Separation was attained on HP column (30 m × 0. 25 mm ID, 0.25 μm) coupled with mass spectrometric detector using electron impact selected ion monitoring. The assay showed an excellent linearity in the concentration range of 0.36–10 μg mL−1 with correlation coefficient (r2) values of 0.999. The intra- and inter-day assay variations for three different concentration levels were less than 10%. The limit of quantification was detected at 0.36 μg mL−1. The method is highly specific, precise, accurate, and reproducible and could also be applied for the determination of pregabalin in human plasma. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.