Positive Electrostatic Therapy of Metastatic Tumors: Selective Induction of Apoptosis in Cancer Cells by Pure Charges Publisher Pubmed



Zandi A^{1, 2} ; Rafizadehtafti S¹ ; Shojaeian F^{1, 3} ; Ali Khayamian M¹ ; Abbasvandi F⁴ ; Faranoush M^{5, 6} ; Anbiaee R⁷ ; Najafikhoshnoo S¹ ; Hoseinpour P⁸ ; Assadi S¹ ; Katebi P¹ ; Davari Sh Z¹ ; Shalileh S¹ ; Salemizadeh Parizi M¹ Show All Authors
Source: Cancer Medicine Published:2021

Abstract

Background: We discovered that pure positive electrostatic charges (PECs) have an intrinsic suppressive effect on the proliferation and metabolism of invasive cancer cells (cell lines and animal models) without affecting normal tissues. Methods: We interacted normal and cancer cell lines and animal tumors with PECs by connecting a charged patch to cancer cells and animal tumors. many biochemical, molecular and radiological assays were carried out on PEC treated and control samples. Results: Correlative interactions between electrostatic charges and cancer cells contain critical unknown factors that influence cancer diagnosis and treatment. Different types of cell analyses prove PEC-based apoptosis induction in malignant cell lines. Flowcytometry and viability assay depict selective destructive effects of PEC on malignant breast cancer cells. Additionally, strong patterns of pyknotic apoptosis, as well as downregulation of proliferative-associated proteins (Ki67, CD31, and HIF-1α), were observed in histopathological and immunohistochemical patterns of treated mouse malignant tumors, respectively. Quantitative real-time polymerase chain reaction results demonstrate up/down-regulated apoptotic/proliferative transcriptomes (P21, P27, P53/CD34, integrin α5, vascular endothelial growth factor, and vascular endothelial growth factor receptor) in treated animal tumors. Expression of propidium iodide in confocal microscopy images of treated malignant tissues was another indication of the destructive effects of PECs on such cells. Significant tumor size reduction and prognosis improvement were seen in over 95% of treated mouse models with no adverse effects on normal tissues. Conclusion: We discovered that pure positive electrostatic charges (PECs) have an intrinsic suppressive effect on the proliferation and metabolism of invasive cancer cells (cell lines and animal models) without affecting normal tissues. The findings were statistically and observationally significant when compared to radio/chemotherapy-treated mouse models. As a result, this nonionizing radiation may be used as a practical complementary approach with no discernible side effects after passing future human model studies. © 2021 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.