An Innovative Method for Synthesis of Imprinted Polymer Nanomaterial Holding Thiamine (Vitamin B1) Selective Sites and Its Application for Thiamine Determination in Food Samples Publisher Pubmed



Alizadeh T¹ ; Akhoundian M¹ ; Ganjali MR^{1, 2}
Source: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences Published:2018

Abstract

Thiamine is a highly hydrophilic vitamin which is insoluble in common organic solvents, utilized traditionally for synthesis of molecularly imprinted polymer. In this work a simple strategy is introduced to overcome the insolubility problem of thiamine and prepare an efficient MIP in chloroform. Thiamine, as a cationic agent, involved in an ion-pair complex with anionic species of trimethylsilyl-propane sulfonate (TPS). The ion-pair was then transferred effectively to apolar solvent of chloroform where the polymerization reaction was conducted to produce nano-sized thiamine imprinted polymer. The experimental conditions such as pH, ionic strength and carrier agent/thiamine mole ratio were optimized to maximize the thiamine amount, transported to chloroform. The characteristics of the polymer were investigated by FT-IR, SEM and Zeta potential analysis techniques. The experimental adsorption isotherms of thiamine onto MIP and non-imprinted polymer (NIP) were determined and well fitted by Langmuir-Freundlich model. Maximum binding capacities of the MIP and NIP materials were obtained as 106.1 and 35.7 μmol g−1, respectively. After investigation and fixing of the experimental conditions on thiamine extraction by the MIP, it was successfully utilized for thiamine extraction from food samples and its determination by fluorimetric method. © 2018