Analysis of Micrornas Expression Changes in Human Breast Cancer Cell Lines Following Exposure to Ionizing Radiation Publisher



Kouhestani SD¹ ; Forouzandeh M¹ ; Aghili M²
Source: International Journal of Radiation Research Published:2018

Abstract

Background: The expression alterations of specific miRNAs were analysed in response to gamma-irradiation in two breast cancer cell lines MCF-7 and MDA-MB- 231 with low and high BCSCs content to design probable strategies for sensitizing breast cancer stem-like cells MDA-MB-231 to radiation. Materials and Methods: The expression levels of hsa-miR-34a, hsa-let-7i and hsa-miR-21 were assessed by QRT-PCR in MCF-7 and MDA-MB-231 breast cancer cells at three time points a/er gamma irradiation using three different doses. Results: The results showed that the over-expression of hsa-miR-34a in MCF-7 cells was much more significant than MDA-MB-231 cells. Furthermore, there was a considerable overexpression of hsa-miR-21 in MDA-MB-231 cells following exposure to ionizing radiation (IR). The hsa-let-7i expression changes were different, depending on radiation dose, time post irradiation and cell type. Conclusion: According to the results, we may be able to sensitize stem-like MDA-MB-231 breast cancer cells to radiation by increasing miR-34a and decreasing miR-21 expression levels simultaneously. © 2018 Novin Medical Radiation Institute.All right reserved.