Development of a Green Single Drop Microextraction Based on Deep Eutectic Solvent and Hplc-Uv for Trace Residue Analysis of Three Frequent-Used Pesticides



Pourhossein M¹ ; Khadem M² ; Omidi F³ ; Heravizadeh OR⁴ ; Shahtaheri SJ^{2, 5}
Source: Iranian Journal of Public Health Published:2023

Abstract

Background: A green sample preparation method named deep eutectic solvent-based single drop microextraction (DES-SDME) was developed and optimized for determining trace metribuzin, dichlorvos, and fenthion. Methods: Two hundred seventy experimental runs were performed, and the optimal values of the five influential factors in the DES-SDME method were determined. The design of the study was based on one factor at a time and the peak area of high-performance liquid chromatography was used as a benchmark for comparing analysis results. Results: After optimizing the effective factors, the linearity range, detection limit and quantification limit of the method were determined by drawing calibration curves for the studied analytes. Conclusion: The results indicated the success of the developed method in obtaining acceptable figures of merit as a green preparation method with accuracy and precision. © 2023 Pourhossein et al.