Identification and Phylogenetic Classification of Fasciola Species Isolated From Sheep and Cattle by Pcr-Rflp in Zabol, in Sistan and Baluchistan Province, Southeast Iran Publisher



Mir S¹ ; Dabirzadeh M² ; Rokni MB^{3, 4} ; Aryaeipour M³ ; Khoshsima Shahraki M² ; Azizi H²
Source: Iranian Journal of Public Health Published:2019

Abstract

Background: The detection of Fasciola species in various geographical regions is essential for health policymak-ing. Here, we aimed to identify livestock (cattle and sheep) related Fasciola genotypes by restriction fragment length polymorphism PCR. Methods: Seventy adult Fasciola flukes were collected from 70 infected livers of 35 cattle and 35 sheep slaugh-tered in Zabol abattoir, outh-east Iran (Jan-Jul 2017). Fasciola species were determined based on molecular fea-tures. For molecular detection, Fasciola ITS1 region was amplified and sequenced. A 700 bp fragment was ampli-fied. These were digested with RasI enzyme. F. hepatica specific fragments were 47, 59, 68, 104, and 370, while those related to F. gigantica had 45, 55, 170, 370. Results: The two main species of F. hepatica and F. gigantica are responsible for fasciolosis in sheep and cattle in our region. From 35 Fasciola isolated from cattle, 3 and 32 were F. hepatica and F. giagantica respectively. From 35 Fasciola isolated from sheep, 4 were F. hepatica and 31 were F. gigantica. Conclusion: All Seventy Fasciola samples from two different hosts (cattle and sheep) were identified as either F. hepatica or F. gigantica by PCR-RFLP. Genotypic variability of Fasciola species was high in our region. It is rec-ommended to assess molecular variation of Fasciola isolates in other host livestock. © 2019, Iranian Journal of Public Health. All rights reserved.