Assessment of Cervical Cancer Molecular-Based Screening Tools; Hpv-Dna Detection Versus E6/E7 Mrna Testing; First Report of a Prospective Cohort Study Among Iranian Women



Mousavi AS^{1, 2, 3} ; Pouryasin A^{4, 5} ; Yarandi F^{2, 3, 6} ; Pirzadeh L^{1, 2, 3} ; Alipour A⁷ ; Khodadad S^{2, 8} ; Pouryasin M³
Source: Iranian Journal of Public Health Published:2020

Abstract

Background: Human papillomavirus (HPV) has been found as the most considerable causes of cervical cancer. Recently, several molecular methods have been introduced to increase the accuracy of the screening pro-grams and decrease the mortality rate. Among these methods, mRNA-based methods have more advantages as they assess the expression level of HPV E6 and E7 oncogenic mRNAs. This study aimed to evaluate the results of HPV RNA-and DNA-based methods among Iranian women population with normal cytology results. Methods: Overall, 4640 women were enrolled referred to the Gynecology Oncology Ward of Vali-e-Asr Hos-pital, private and academic clinics, Tehran, Iran from Jan 2016 to Apr 2018. To assess the HPV-DNA infection INNO-LiPA® HPV Genotyping Extra-II kit was used. For HPV-RNA assessment, Aptima HPV Assay and in house HPV-RNA genotyping methods were applied. Results: The positivity rates of HPV infection according to DNA-and RNA-based methods were 18.0% and 11.2%, respectively (P<0.001). The positive predictive value, negative predictive value, specificity and sensitivity of DNA-based method in contrast with RNA-based method were 59.2% (56.6-61.6), 99.4% (99.0-99.6), 91.7% (90.8-92.6) and 95.2% (93.0-96.9) respectively. Conclusion: At the present study for prognosis of cervical cancer, RNA-based method seemed to be more specific in contrast to DNA-based method. Patient follow up and further studies will be conducted in order to clarify the clinical sensitivity and specificity of the two methods. © 2020, Iranian Journal of Public Health. All rights reserved.