A Qhnmr Method for Simultaneous Quantification of Terpenoids From Ferula Ovina (Boiss.)Boiss Roots Publisher Pubmed



Farhadi F¹ ; Soltani S² ; Saberi S³ ; Iranshahi M⁴
Source: Journal of Pharmaceutical and Biomedical Analysis Published:2019

Abstract

Ferula ovina (Boiss.)Boiss is one of the most important endemic medicinal plants in Iran, which has three main terpenoid compounds including ferutinin, stylosin and tschimgine. Ferutinin is the strongest natural phytoestrogen that has agonistic activity on estrogen receptors, particularly α-receptors. To determine the amount of ferutinin in F. ovina roots, we firstly used HPLC-UV method. In the HPLC method, the resolution of ferutinin from the two other compounds, stylosin and tshimgine, was poor. Therefore, we decided to use qHNMR method for simultaneous quantification of ferutinin, stylosin and tshimgine in the plant roots. Quantitative 1 H-NMR (qHNMR)was carried out based on the relative ratio of signal integration of each compound [(H-1 for tschimgine (δ H 4.94–5.03), OCH 3 for stylosin (δ H 3.8), and H-9 for ferutinin (δ H 5.58)]to certain amount of the internal standard dimethyl sulfone (DMSO 2 ). The qHNMR method showed good precision (intra-day RSD ≤ 2.31%, inter-day RSD ≤ 2.72%), linearity (in the ranges of 1.3–10.41, 1.2–9.7 and 1.1–9.02 mg/mL with correlation coefficients at 0.9991), repeatability (RSD ≤ 2.99%)and stability (RSD ≤ 2.4%)for the quantification of the compounds. This work confirmed that qHNMR represents a feasible alternative to high-performance liquid chromatography based methods for simultaneous quantification of ingredients in plant extracts. © 2019