Inhibition of Biofilm Formation of Staphylococcus Aureus Strains Through Zno/Zeolite Nanocomposite and Its Cytotoxicity Evaluation Publisher



Partoazar A¹ ; Bideskan FR² ; Partoazar M³ ; Talaei N⁴ ; Dallal MMS^{2, 5}
Source: BioNanoScience Published:2020

Abstract

Staphylococcus aureus biofilm formation through its binding to the surface of medical devices is a common and serious problem in clinic. This study aimed to evaluate the antibiofilm activity of zinc oxide containing zeolite nanocomposite (ZnO/ZeoNC) against standard and clinical strains of S. aureus and to assess its cytotoxicity on the human cell. The biofilm formation of S. aureus strains was exposed to ZnO/ZeoNC and then SEM imaging was provided for the morphology investigation of bacterial biofilm formation on polyethylene surface. Cell cytotoxicity of nanocomposite was assessed on HEp-2 cells during a 72-h exposure to concentrations of 44 μg/ml up to 350 μg/ml of Zn containing ZnO/ZeoNC. Results indicated that ZnO/ZeoNC had a significant (P < 0.05) antibiofilm action against S. aureus strains in sublethal dose of the nanocomposite. The influence of ZnO/ZeoNC on S. aureus biofilm revealed the elimination of the bacteria biofilm from the abiotic surface during 24-h cultivation. Furthermore, cell cytotoxicity of ZnO/ZeoNC exhibited an increasing trend that was dependent on the elevation of the zinc level. In conclusion, the current data suggest the usage of ZnO/ZeoNC in a safe and non-toxic manner against infectious due to staphylococcal colonization. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.