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Differentiation of Neural Crest Stem Cells From Nasal Mucosa Into Motor Neuron-Like Cells Publisher Pubmed



Bagher Z1 ; Kamrava SK1 ; Alizadeh R1 ; Farhadi M1 ; Absalan M2 ; Falah M1 ; Faghihi F3 ; Zaresadeghi A4 ; Komeili A5
Authors

Source: Journal of Chemical Neuroanatomy Published:2018


Abstract

Cell transplantation is a potential therapeutic approach for repairing neuropathological and neurodegenerative disorders of central nervous system by replacing the degenerated cells with new ones. Among a variety of stem cell candidates to provide these new cells, olfactory ectomesenchymal stem cells (OE-MSCs) have attracted a great attention due to their neural crest origin, easy harvest, high proliferation, and autologous transplantation. Since there is no report on differentiation potential of these cells into motor neuron-like cells, we evaluated this potential using Real-time PCR, flowcytometry and immunocytochemistry after the treatment with differentiation cocktail containing retinoic acid and Sonic Hedgehog. Immunocytochemistry staining of the isolated OE-MSCs demonstrated their capability to express nestin and vimentin, as the two markers of primitive neuroectoderm. The motor neuron differentiation of OE-MSCs resulted in changing their morphology into bipolar cells with high expression of motor neuron markers of ChAT, Hb-9 and Islet-1 at the level of mRNA and protein. Consequently, we believe that the OE-MSCs have great potential to differentiate into motor neuron-like cells and can be an ideal stem cell source for the treatment of motor neuron-related disorders of central nervous system. © 2018 Elsevier B.V.
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