Formulation, in Vitro Evaluation and Kinetic Analysis of Chitosan–Gelatin Bilayer Muco-Adhesive Buccal Patches of Insulin Nanoparticles Publisher Pubmed



Mahdizadeh Barzoki Z¹ ; Emamdjomeh Z^{1, 2} ; Mortazavian E³ ; Akbar Moosavimovahedi A⁴ ; Rafiee Tehrani M³
Source: Journal of Microencapsulation Published:2016

Abstract

The present study was performed to optimise the formulation of a muco-adhesive buccal patch for insulin nanoparticles (NPs) delivery. Insulin NPs were synthesised by an ionic gelation technique using N-di methyl ethyl chitosan cysteine (DMEC-Cys) as permeation enhancer biopolymer, tripolyphosphate (TPP) and insulin. Buccal patches were developed by solvent-casting technique using chitosan and gelatine as muco-adhesive polymers. Optimised patches were embedded with 3 mg of insulin-loaded NPs with a homogeneous distribution of NPs in the muco-adhesive matrix, which displayed adequate physico-mechanical properties. The drug release characteristics, release mechanism and kinetics were investigated. Data fitting to Peppas equation with a correlation coefficient indicated that the mechanism of drug release followed an anomalous transport that means drug release was afforded through drug diffusion along with polymer erosion. In vitro drug release, release kinetics, physical and mechanical studies for all patch formulations reflected the ideal characteristics of this buccal patch for the delivery of insulin NPs. © 2016 Informa UK Limited, trading as Taylor & Francis Group.