Formulation and Evaluation of Targeted Nanoparticles for Breast Cancer Theranostic System Publisher Pubmed



Dadras P¹ ; Atyabi F^{1, 2} ; Irani S³ ; Mamani L⁴ ; Foroumadi A⁵ ; Mirzaie ZH⁶ ; Ebrahimi M⁷ ; Dinarvand R^{1, 2}
Source: European Journal of Pharmaceutical Sciences Published:2017

Abstract

Theranostic polymeric NPs developed for both cancer diagnosis and cancer therapy. This multifunctional polymeric vehicle was prepared by a single emulsion evaporation method, using carboxyl-terminated PLGA. LHRH as a targeting moiety, was conjugated to the surface of polymeric carrier by applying polyethylene glycol. The results indicated that the diameter of NPs was ~ 185.4 ± 4.6 nm as defined by DLS. The entrapment efficacy of docetaxel, silibinin, and SPIONs was 84.6 ± 4.1%, 80.6 ± 2.7%, and 77.9 ± 4.3%, respectively. The NPs showed a triphasic in-vitro drug release pattern. MTT assay was done on two cell lines, MCF-7 and SKOV-3. Enhanced cellular uptake ability of the targeted NPs to MCF-7 was evaluated in-vitro by confocal laser scanning microscopy. The results indicated that compared to non-targeted NPs, the LHRH targeted NPs had significant efficacy at IC50 concentration. The effect of the NPs on VEGF expression in MCF-7 and SKOV-3 cells was investigated by Real-Time PCR method. VEGF mRNA level expression in MCF-7 cell line reduced by 83% in comparison to control cell line. The designed NPs can be used as promising multifunctional platform for detection and targeted drug delivery in breast cancer. © 2016 Elsevier B.V.