Design and Synthesis of Mucoadhesive Nanogel Containing Farnesol: Investigation of the Effect on Hwp1, Sap6 and Rim101 Genes Expression of Candida Albicans in Vitro Publisher Pubmed



Nikoomanesh F¹ ; Roudbarmohammadi S¹ ; Khoobi M² ; Haghighi F³ ; Roudbary M⁴
Source: Artificial Cells# Nanomedicine and Biotechnology Published:2019

Abstract

The evolution of drug resistance of Candida species to conventional antifungal agents has been a major medical challenge worldwide; attempt to use the potential antifungal agents with appropriate therapy efficacy and minimum effects is considerably growing. This study was conducted to evaluate the use of nanogel as a nanocarrier for pharmaceutical application of farnesol. The nanogels were synthetized using alginate (AL) and chitosan (CS) polymers containing 300 µM of farnesol in the nano-range 42–70 nm size. In vitro release studies indicated that release of farnesol from CS and AL nanogels was as 58% and 37%, respectively. Chitosan nanogel showed more in inhibitory zone as compared to AL nanogel (9 mm). Also, cytotoxicity assay showed no significant difference between control and treatment groups (p>.05). Finally, the effect of nanogels on genes expression of HWP1, SAP6 and Rim101 in Candida albicans ATCC10231 was assessed using real-time polymerase chain reaction (PCR). Expression of HWP1 and SAP6 genes in C. albicans treated with CS nanogel was significantly decreased (p<.01). In general, the obtained finding showed that, CS nanogel contains farnesol with proper antifungal activity and as a new approach used in pharmaceutical applications against C. albicans; however, more studies in vitro and in vivo are needed in the future. © 2019, © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.