Electrochemical Immunosensor for Determination of Cardiac Troponin I Using Two-Dimensional Metal-Organic Framework/Fe3o4–Cooh Nanosheet Composites Loaded With Thionine and Pctab/Des Modified Electrode Publisher



Ahmadi A^{1, 2} ; Khoshfetrat SM³ ; Mirzaeizadeh Z¹ ; Kabiri S^{1, 2} ; Rezaie J² ; Omidfar K^{1, 2}
Source: Talanta Published:2022

Abstract

Cardiac troponin-I (CTnI) is one of the most popular biomarkers which can be utilized for the diagnosis and control of acute myocardial infarction in clinical practice. Here, a sandwich-type electrochemical immunosensor has been established using the zinc-based metal-organic framework/Fe3O4–COOH/thionine labeled anti-CTnI monoclonal antibody (Ab1-Zn-MOF/Fe3O4–COOH/Thi) nanocomposite as signaling molecule and a polymer film of cetyltrimethylammonium bromide (pCTAB) in the presence of choline chloride-urea deep eutectic solvent (DES) and anti-CTnI polyclonal antibody (Ab2) as immobilization substance of detecting surface. The porous ultrathin layers of Zn-MOF nanosheets successfully prepare a well-defined structure for Fe3O4–COOH electrocatalyst and Thi within a certain two dimensional (2D) regions, which enhances electrochemical reduction of Thi. The Ab1-Zn-MOF/Fe3O4–COOH/Thi nanocomposites were introduced to CTnI in the specimen and on the surface of pCTAB/DES-Au-SPE quantitative determination of CTnI was achieved using differential pulse voltammetry after sandwiching the CTnI target between Ab1-nanocomposite and Ab2 which was encapsulated into the pCTAB/DES-Au-SPE. This immunosensor indicated the appropriate assay performance for CTnI with the detection range of 0.04 ng mL−1 to 50 ng mL−1 and the limit of detection of 0.0009 ng mL−1. This study provides convenient plan for sensitive detection of bioanalytes and opens a path for the establishment of user-friendly and cost-effective device. © 2021 Elsevier B.V.