Plant-Based Dietary Indices Association With Appetite, Appetite Regulating Peptides and Gut Microbiota in Healthy Women: A Cross-Sectional Study Publisher Pubmed



Barkhidarian B¹ ; Soveid N¹ ; Samadi M¹ ; Lesani A¹ ; Aghakhani A² ; Yekaninejad MS² ; Saedisomeolia A^{3, 4} ; Karbasian M⁵ ; Siadat SD⁵ ; Mirzaei K¹
Source: European Journal of Nutrition Published:2025

Abstract

Purpose: Plant-based diets are associated with improved appetite regulation. Moreover, gut microbiota has been linked to appetite. The present study aims to determine the association between plant-based dietary indices (PDIs) and appetite-regulating peptides (fasting level of Leptin, GLP-1, and ghrelin) and gut microbiota profile in healthy women. Furthermore, the potential covariate role of gut microbiota in the association between PDIs and appetite is investigated. Methods: This cross-sectional study was conducted on 91 healthy women (18–50 years). Body composition, anthropometric indices, dietary intake, PDIs, subjective appetite, appetite-regulating peptides (fasting level of leptin, GLP-1, and ghrelin), physical activity, sleep quality, and gut microbiota profile were evaluated. Results: There was a significant inverse association between scores of PDI and healthful plant-based dietary index (hPDI) with leptin and IL-6 (p < 0.05). A higher hPDI was associated with a lower GLP-1 concentration (p = 0.04). Additionally, uPDI (unhealthful plant-based diet index) was inversely associated with satiety (p = 0.02) and positively associated with hunger (p = 0.02). Moreover, higher PDI was associated with higher Prevotella abundance (p = 0.01). Our findings suggest that Firmicutes and the Firmicutes/Bacteroidetes ratio might be involved in the associations between hPDI and both leptin and GLP-1 levels. Additionally, A. muciniphila may play a role in the association between hPDI and GLP-1 levels as a relevant microbial factor. However, the potential mediating effects of these bacteria should be investigated in future studies. Conclusion: We indicated an inverse association between higher PDI and hPDI scores with appetite-related hormones and IL-6. Moreover, higher uPDI was related to appetite sensation and a higher PDI score increased the abundance of Prevotella. The association between hPDI scores and appetite-regulating hormones may be influenced by the gut microbiota. Considering lower levels of fasting GLP-1 in relation with hPDI, it appears further evaluation of the postprandial state of GLP-1 in cohort studies or intervention trials is also warrented to better elucidate their association. © Springer-Verlag GmbH Germany, part of Springer Nature 2025.