Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share By
Mgmt Gene Rs1625649 Polymorphism in Iranian Patients With Brain Glioblastoma: A Case Control Study Publisher



Safaei R1 ; Mojtahedi H2, 3 ; Hanaei S4 ; Razavi A5 ; Esmaeili M5 ; Sadr M2 ; Rezaei A5 ; Edalatfar M6 ; Kashani HK6 ; Sadeghinaini M7 ; Darbeheshti F8 ; Gharehdaghi J9 ; Forouzesh M9 ; Ebrahimi A10 Show All Authors
Authors
  1. Safaei R1
  2. Mojtahedi H2, 3
  3. Hanaei S4
  4. Razavi A5
  5. Esmaeili M5
  6. Sadr M2
  7. Rezaei A5
  8. Edalatfar M6
  9. Kashani HK6
  10. Sadeghinaini M7
  11. Darbeheshti F8
  12. Gharehdaghi J9
  13. Forouzesh M9
  14. Ebrahimi A10
  15. Rezaei N3, 5, 11

Source: Avicenna Journal of Medical Biotechnology Published:2023


Abstract

Glioblastoma (GBM) is the most common and aggressive primary malignant brain tumor with poor prognosis and high potential of dispersion to other brain tissues in adult. Effective and modern choices of treatment including chemotherapy with alkylating agents marginally extend survival of GBM. However, alkylating agents can lead to highly harmful mismatch during DNA replication causing apoptosis and cell death. Accordingly, O6-Methylguanine-DNA methyltransferase (MGMT) removes alkyl adducts, thereby causing resistance to alkylating drugs. Single-Nucleotide Polymorphisms (SNPs) in MGMT promoter region may play a role in the regulation of MGMT expression and prediction of glioma development risk. In order to evaluate the clinical significance of rs1625649 SNP in the MGMT promoter region of glioblastoma, genomic DNA from a series of 54 patients with GBM and 50 healthy individuals in Iranian population were collected for tetra ARMS PCR amplification. None of the A or C alleles were associated with tumor occurrence, the AA genotype was more frequent in healthy subjects, and the AC genotype was 4.6 times more common in patients with GBM. The longest survival time was observed in the CC genotype; however, this difference was not statistically significant. On the other hand, homozygous rs1625649 (AA genotype) was significantly associated with a better survival than the cases with heterozygous rs1625649 (CA genotype) or wild type rs1625649 (CC genotype), predicting better response to temozolomide-based chemotherapy. © 2023, Avicenna Journal of Medical Biotechnology. All rights reserved.
Other Related Docs
10. An Overview of Neuro-Oncology Research and Practice in Iran, Three Years With the Nosc Initiative, International Journal of Clinical and Experimental Medicine (2015)