Resistance to Chlorhexidine and Benzalkonium Chloride in E. Coli St131, A. Baumannii, and P. Aeruginosa Isolates Publisher Pubmed



Farahani HE¹ ; Asgharzadeh S² ; Golmoradizadeh R¹ ; Akrami S² ; Ghaznavirad E³ ; Mirkalantari S¹ ; Masjedianjazi F¹
Source: Clinical Laboratory Published:2024

Abstract

Background: The goal was to assess the antimicrobial efficacy of two commonly used biocides, chlorhexidine, and benzalkonium chloride, against MDR isolates of Pseudomonas aeruginosa, Acinetobacter baumannii, and Escherichia coli ST131, as well as the prevalence of resistance genes. Methods: MIC of chlorhexidine and benzalkonium chloride and their effects on both the planktonic phase and biofilm were determined. Finally, the presence of genes responsible for resistance to quaternary ammonium compounds was investigated by PCR. Results: No significant relationship was observed between the presence of resistance genes and different concentrations of quaternary ammonium compounds (benzalkonium chloride). There was no association between biofilm formation and the presence of resistance genes. Conclusions: Chlorhexidine digluconate and benzalkonium chloride at appropriate concentrations could prevent biofilm formation. © 2024 Verlag Klinisches Labor GmbH. All rights reserved.