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The Effect of Indocyanine Green Antimicrobial Photothermal/Photodynamic Therapy on the Expression of Bcl-2 and Bax Messenger Rna Levels in Human Gingival Fibroblast Cells; [Влияние Антимикробной Фототермической / Фотодинамической Терапии Индоцианиновым Зелёным На Экспрессию Уровней Bcl-2 И Bax В Матричной Рнк В Клетках Фибробластов Десны Человек] Publisher Pubmed



Pourhajibagher M1 ; Gharesi S2 ; Chiniforush N3 ; Bahador A4
Authors

Source: Folia Medica Published:2020


Abstract

Background: Antimicrobial photothermal/photodynamic therapy (PTT/PDT) with indocyanine green (ICG) is an adjuvant therapeutic approach in the treatment of periodontitis. To explore whether PTT/PDT with ICG causes cell death by apoptosis in human gingival fibroblast (HGF) cells, BAX and BCL-2 genes expression as key events for apoptosis were evaluated in this study. Materials and methods: HGF cells were treated with: 1) different concentrations (500-2000 μg/m L) of ICG alone, 2) Diode laser irradiation alone with a fluency of 39.06 J/cm2; 3) PTT/PDT combined different concentrations (500-2000 μg/mL ) of ICG with an 808 nm diode laser with a fluency of 39.06 J/cm2,and 4) controls (untreated cells). After that, BAX and BCL-2 messenger RNA levels were evaluated by real-time quantitative reverse transcription PCR. Results: PTT/PDT with 500 μg/mL of ICG caused significant increases in the expression of the BAX gene, with an 8.5-fold increase, which was approximately 7- and 8.5-fold higher than PTT/PDT with ICG for 1500 and 2000 μg/mL of ICG, respectively, indicating induction of apoptosis in HGF cells. ICG (in different test concentrations), diode laser, and PTT/PDT with ICG (1500 and 2000 μg/mL of ICG) treatment displayed insignificant increases in expression levels of BAX (all p >0.05). Our experiments showed an insignificant increase (1.1-1.6-fold) in the expression of BCL-2 after ICG, diode laser, and PTT/PDT with ICG treatment (all p >0.05). Conclusions: This study suggests that various concentration of ICG can be the diverse expression of BAX responses to PTT/PDT on HGF cells. © 2020 The Author(s).
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