Role of Stromal Derived Factor-1A (Sdf-1A) for Spermatogenesis of Busulfan-Injured Rats Publisher Pubmed



Khanlarkhani N¹ ; Mortezaee K² ; Amidi F¹ ; Kharazinejad E³ ; Beyer C⁴ ; Baazm M⁵ ; Pasbakhsh P¹ ; Pazhohan A¹ ; Sobhani A¹ ; Zendedel A⁴
Source: Reproductive Toxicology Published:2017

Abstract

SDF-1a is a member of CXC chemokine family that plays a crucial role in stem cell migration, cell apoptosis and development. The role of intra-scrotal administration of SDF-1a in spermatogenesis of busulfan-treated rats was investigated in this study. Two injections of busulfan (15 mg/kg) with a 14 days interval between were given intraperitoneally to male Wistar rats. Rats were then treated for seven days with 500 ng/mL SDF-1a. Real-time PCR and immunohistochemistry were performed for evaluation of various cell markers for proliferation and spermatogenesis, and sperm parameters were assessed. In the SDF-1a group, there was a significant increase in testis weight, sperm count and viability. DAZL, DDX4, and TP2 showed increased expression levels in the SDF-1a group. PCNA and BrdU revealed highest expression rates in the SDF-1a group (p ≤ 0.0001). These findings showed the protective role of SDF-1a in busulfan-induced testis injury most likely through stimulation of SSCs proliferation. © 2017 Elsevier Inc.