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A New Elisa Kit Based on Antigenic Epitopes for Diagnosing Brucella Abortus Publisher



Khodabakhsh T1 ; Arabi A1 ; Pakzad P1 ; Gheflat S2 ; Bahreinipour A3 ; Bandehpour M2, 4
Authors

Source: Korean Journal of Microbiology and Biotechnology Published:2019


Abstract

Brucellosis is one of the most important zoonotic diseases that lead to a great amount of economic losses. Prevention and diagnosis are both necessary to eradicate this disease. The identification and evaluation of different antigens of Brucella spp. play a key role in the progress of diagnostic programs. In this study, we designed, produced, and evaluated a 24-kDa polypeptide containing antigenic epitopes of VirB2, 3, and 9 of Brucella abortus for use with the ELISA kit. The produced polypeptide is appropriate for diagnosing brucellosis in bovines by a laboratory diagnostic kit, with 100% sensitivity and 97.5% specificity. © 2019 The Korean Society for Microbiology and Biotechnology.
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