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Upregulated Long Non-Coding Rnas Tmpo-As1, Ddx11-As1, and Pole Gene Expression Predict Poor Prognosis in Head and Neck Squamous Cell Carcinoma (Hnscc) Publisher



Mokhtarinejad M1 ; Pirhoushiaran M1 ; Heidarzadehpilehrood R2 ; Hesami S3 ; Azmoudehardalan F4 ; Farahani AS3
Authors

Source: Gene Reports Published:2024


Abstract

Background: Head and neck squamous cell carcinoma (HNSCC) is prevalent and highly aggressive. HNSCC pathogenesis is characterized by the presence of long non-coding RNAs (lncRNAs) that lead to abnormal cellular functions such as proliferation, migration, and invasion. Methods: This study examined the expression levels of lncRNAs TMPO-AS1, DDX11-AS1, and the POLE gene in 100 HNSCC tumors and normal adjacent tumors (NATs). The analysis was performed using quantitative reverse transcription polymerase chain reaction (RT-qPCR) on fresh-frozen samples. Results: In HNSCC samples, tumor tissue exhibited markedly elevated levels of POLE expression compared to NATs (p < .001, 95 % CI: 2.410–3.170 vs. 0.9834–1.549). DDX11-AS1 expression was also significantly higher in HNSCC samples than in NATs (p < .001, 95 % CI: 1.879–3.250 vs. 1.029–1.731). Similarly, TMPO-AS1 levels were significantly elevated in HNSCC samples compared to NATs (p < .001, 95 % CI: 1.903–2.639 vs. 0.894–1.329). ROC curve diagnostic analyses revealed AUC values of 0.838 (95 % CI: 0.761–0.915) for POLE, 0.689 (95 % CI: 0.585–0.793) for DDX11-AS1, and 0.808 (95 % CI: 0.724–0.893) for TMPO-AS1. Notably, a substantial association was found between tumor grade and human papillomavirus levels with POLE and TMPO-AS1. Additionally, significant associations were observed between tumor stage and metastasis with DDX11-AS1 and TMPO-AS1 levels. Our findings further investigate a remarkable association between the combined effect of smoking and HPV positive infections and expression levels of POLE, DDX11-AS1, and TMPO-AS1. Our final results align with those from The Cancer Genome Atlas (TCGA), which included 520 HNSCC cases and 44 controls. Conclusions: Our data emphasize the potential significance of DDX11-AS1, TMPO-AS1, and POLE in the poor prognosis of HNSCC, indicating their contribution to tumor growth and progression. © 2024
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