Anti-Aging Potential of Callus Culture: Mechanisms, Efficacy and Future Trends Publisher



Sharafi A M ; Khajeei A ; Navroodi M H Z ; Mokari S ; Najafi S H ; Argha M ; Shahmirzadi F T ; Asgharian P ; Gholikhani T
Source: Journal of Cosmetic Dermatology Published:2026

Abstract

Background: Callus culture technology revolutionizes skin antiaging research, offering a sustainable and efficient method for producing bioactive compounds with potent geroprotective properties. This plant tissue and cell culture technique enables the controlled biosynthesis of beneficial phytochemicals while mitigating the variability and contamination risks inherent in traditional plant sources. By manipulating phytohormonal pathways, researchers can increase the yield of secondary metabolites that possess therapeutic and cosmetic efficacy, aligning with ethical standards of environmental stewardship. Methods: A thorough literature search was carried out to assess the anti-aging potential of callus culture using the search terms (callus culture OR plant stem cells) AND (anti-aging OR skin aging OR photoaging) AND (skin OR skincare) AND (bioactive compounds OR phytochemicals OR antioxidants). Additional searches included (mechanisms OR collagen OR anti-inflammatory OR wound healing) AND (cosmetic OR cosmeceutical OR skin rejuvenation). Relevant studies were also identified by screening the reference lists of the retrieved articles. Results: Numerous studies have demonstrated the efficacy of callus cultures derived from various plant species, such as Malus domestica (apple), Oryza sativa (rice), Centella asiatica, and Aster yomena, in generating phytochemicals rich in antioxidants and anti-inflammatory agents. These compounds promote skin health and combat oxidative stress. In vitro cultures have shown the ability to increase keratinocyte proliferation, stimulate collagen synthesis, inhibit elastase activity, and reduce the levels of inflammatory markers associated with skin aging. Furthermore, the callus culture extracts mitigate the adverse effects of oxidative stress and inflammation, major contributors to skin aging. This renders them invaluable sources of geroprotective agents suitable for cosmeceutical and pharmaceutical applications. The growing consumer demand for natural ingredients has led to a notable market shift toward the use of botanical preparations for anti-aging solutions. Conclusion: In conclusion, the integration of callus culture techniques offers a novel approach for the development of antiaging skincare products. By leveraging the inherent ability of plant cells to synthesize bioactive metabolites, researchers and cosmetic scientists can create effective and sustainable formulations that meet the demand for natural skincare solutions. Discussion: It should be emphasized that the evidence presented in this review shows mixed conclusions: the majority of reported effects derive from acellular biochemical assays (e.g., DPPH, ABTS, ORAC) and cell-based studies in keratinocytes and fibroblasts, whereas controlled human clinical data remain limited to a small number of studies. Accordingly, the geroprotective claims presented here should be interpreted in light of this evidence hierarchy, and the conclusions are framed as promising but preliminary pending larger clinical validation. © 2026 The Author(s). Journal of Cosmetic Dermatology published by Wiley Periodicals LLC.