Chelator-Free Radiolabeling of Dextran With 68Ga for Pet Studies Publisher



Gholipour N¹ ; Akhlaghi M² ; Kheirabadi AM² ; Beiki D² ; Geramifar P² ; Yousefnia H³ ; Mazidi M³
Source: Journal of Radioanalytical and Nuclear Chemistry Published:2017

Abstract

The possibility of 68Ga-dextran complex formation was analyzed in an HEPES-buffered (pH = 4.5) as well as in an alkaline (pH = 9) solution. The result outlined that the alkaline solution was more efficient to achieve a radiolabeling efficiency of 99% for a 5 mg ml−1 dextran solution (10 min, 90 °C). 68Ga-dextran was stable 88, 71 and 86% after 2 h at storage and toward cysteine solution and human serum, respectively. The highest ROI derived %ID g−1 was found within the lungs, followed by the blood, liver, and bladder. Furthermore, a significant uptake was observed in the cartilage tissue. 68Ga-dextran excreted more by the urinary and less by the biliary pathways. © 2017, Akademiai Kiado, Budapest, Hungary.