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Comparison of Three Cost Effective Staining Methods for Detection of Pneumocystis Jirovecii Publisher



Homayouni MM1 ; Rostami A2 ; Gholizadeh H1 ; Mehbod ASA1 ; Ebrahimi M2 ; Mehravar S3
Authors

Source: Journal of Parasitic Diseases Published:2017


Abstract

Pneumocystis pneumonia due to Pneumocystis jirovecii infection is an emerging health problem not only for HIV-infected patients but also for other immunocompromised patients in many countries. We compared Gomori methenamine silver (GMS), Toluidine Blue O (TBO) and Giemsa staining methods using standard procedures. The sensitivity and specificity of GMS were 100 %. The sensitivity and specificity of TBO were 96 and 100 %, respectively. The sensitivity and specificity of Giemsa stain were 84 and 90 %, respectively. Only GMS had positive and negative predictive values of 100 % while PPV and NPV for TBO were 100 and 90.9 %, and for Giemsa stain were 95.4 and 69.2 %, respectively. Therefore, our results suggest that if TBO or Geimsa stains are used as the primary staining methods in a clinical laboratory, then confirmation with a GMS staining method should be performed to increase the sensitivity and specificity of the final test result. © 2016, Indian Society for Parasitology.