Molecular Typing of Cytotoxin-Producing Klebsiella Oxytoca Isolates by 16S-23S Internal Transcribed Spacer Pcr

Molecular Typing of Cytotoxin-Producing Klebsiella Oxytoca Isolates by 16S-23S Internal Transcribed Spacer Pcr Publisher

Soltan Dallal MM^{1, 2} ; Validi M^{3, 4} ; Douraghi M^{2, 4} ; Bakhshi B⁵

Source: New Microbes and New Infections Published:2019

Abstract

Cytotoxin is one of the important pathogenic factors, which plays a role in the virulence of Klebsiella oxytoca. The aim of this study was to investigate molecular typing of clinical isolates of the cytotoxin-producing K. oxytoca using internal transcribed spacer (ITS)PCR. A total of 75 isolates of K. oxytoca were isolated from clinical samples; they were verified as K. oxytoca by standard microbiological tests and PCR. Production of toxin determines the cytotoxic effects on HEp-2 cells. The genetic diversity of isolates of the cytotoxin-producing K. oxytoca were defined by ITS-PCR. Of all the isolates investigated, five K. oxytoca strains isolated from stool cultures, two strains from blood samples, one strain from a wound and one strain isolated from urine had cytotoxic effects on HEp-2 cells. The ITS-PCR patterns showed genetic diversity among cytotoxin-producing isolates. The ITS-PCR method had good discriminatory power; performance of this method and interpretation of the results were easy and repeatable. Five genetic diversity patterns were identified by ITS-PCR. © 2019 The Author(s)

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Masoumeh Douraghi (5)

Mohammad Mehdi Soltan Dallal (4)

Style	Citing Format
MLA	Soltan Dallal MM, et al.. "Molecular Typing of Cytotoxin-Producing Klebsiella Oxytoca Isolates by 16S-23S Internal Transcribed Spacer Pcr." New Microbes and New Infections, vol. 30, no. , 2019, pp. -.
APA	Soltan Dallal MM, Validi M, Douraghi M, Bakhshi B (2019). Molecular Typing of Cytotoxin-Producing Klebsiella Oxytoca Isolates by 16S-23S Internal Transcribed Spacer Pcr. New Microbes and New Infections, 30(), -.
Chicago	Soltan Dallal MM, Validi M, Douraghi M, Bakhshi B. "Molecular Typing of Cytotoxin-Producing Klebsiella Oxytoca Isolates by 16S-23S Internal Transcribed Spacer Pcr." New Microbes and New Infections 30, no. (2019): -.
Harvard	Soltan Dallal MM et al. (2019) 'Molecular Typing of Cytotoxin-Producing Klebsiella Oxytoca Isolates by 16S-23S Internal Transcribed Spacer Pcr', New Microbes and New Infections, 30(), pp. -.
Vancouver	Soltan Dallal MM, Validi M, Douraghi M, Bakhshi B. Molecular Typing of Cytotoxin-Producing Klebsiella Oxytoca Isolates by 16S-23S Internal Transcribed Spacer Pcr. New Microbes and New Infections. 2019;30():-.
BibTex	@article{ author = {Soltan Dallal MM and Validi M and Douraghi M and Bakhshi B}, title = {Molecular Typing of Cytotoxin-Producing Klebsiella Oxytoca Isolates by 16S-23S Internal Transcribed Spacer Pcr}, journal = {New Microbes and New Infections}, volume = {30}, number = {}, pages = {-}, year = {2019} }
RIS	TY - JOUR AU - Soltan Dallal MM AU - Validi M AU - Douraghi M AU - Bakhshi B TI - Molecular Typing of Cytotoxin-Producing Klebsiella Oxytoca Isolates by 16S-23S Internal Transcribed Spacer Pcr JO - New Microbes and New Infections VL - 30 IS - SP - EP - PY - 2019 ER -

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