Optimization of Culture Conditions for Enrichment of Saccharomyces Cerevisiae With Dl-Α-Tocopherol by Response Surface Methodology



Amiri MM¹ ; Fazeli MR² ; Amini M³ ; Roodbari NH¹ ; Samadi N²
Source: Iranian Journal of Pharmaceutical Research Published:2017

Abstract

Designing enriched probiotic supplements may have some advantages including protection of probiotic microorganism from oxidative destruction, improving enzyme activity of the gastrointestinal tract, and probably increasing half-life of micronutrient. In this study Saccharomyces cerevisiae enriched with dl-α-tocopherol was produced as an accumulator and transporter of a lipid soluble vitamin for the first time. By using one variable at the time screening studies, three independent variables were selected. Optimization of the level of dl-α-tocopherol entrapment in S. cerevisiae cells was performed by using Box-Behnken design via design expert software. A modified quadratic polynomial model appropriately fit the data. The convex shape of three-dimensional plots reveal that we could calculate the optimal point of the response in the range of parameters. The optimum points of independent parameters to maximize the response were dl-α-tocopherol initial concentration of 7625.82 μg/mL, sucrose concentration of 6.86 % w/v, and shaking speed of 137.70 rpm. Under these conditions, the maximum level of dl-α-tocopherol in dry cell weight of S. cerevisiae was 5.74 μg/g. The resemblance between the R-squared and adjusted R-squared and acceptable value of C.V% revealed acceptability and accuracy of the model. © 2017 by School of Pharmacy Shaheed Beheshti University of Medical Sciences and Health Services.