Soluble Guanylate Cyclase Isoenzymes: The Expression of Α1, Α2, Β1, and Β2 Subunits in the Benign and Malignant Breast Tumors Publisher Pubmed



Mohammadookhorasani M¹ ; Karami Tehrani F¹ ; Atri M²
Source: Journal of Cellular Physiology Published:2020

Abstract

Soluble guanylate cyclase (sGC) encompasses α and β subunits. This study examined the expression of α1, α2, β1, and β2 subunits in the malignant and benign breast tumors using the Western blot analysis. Both benign and malignant tumors showed a significantly higher expression of the α1 subunit in comparison with normal tissues (p < 0.0001). In contrast, the expression of α2 and β2 sGC were significantly lower in these tumors than normal tissues (p <.0015 and p <.001, p <.007 and p <.0001, respectively). The expression level of α1 sGC was significantly correlated with ER + PR+ (p <.0001). A significant correlation was also detected for sGC-α1 and -α2 expression with c-erbB2-negative status (p <.01). However, the expression level of sGC was not associated with tumor stage, tumor grade, or other clinicopathological features. In conclusion, as the expression of α1 sGC is upregulated and α2 and β2 sGC are downregulated in malignant breast tumors. Variations in the expression of sGC isoenzymes may be suggested as an indicator to confirm the enzyme antitumor activity. © 2019 Wiley Periodicals, Inc.