Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share By
Enhanced Cellular Cytotoxicity and Antibacterial Activity of 18-Β-Glycyrrhetinic Acid by Albumin-Conjugated Plga Nanoparticles Publisher Pubmed



Darvishi B1, 2 ; Manoochehri S1, 2 ; Esfandyarimanesh M2, 3 ; Samadi N4 ; Amini M5 ; Atyabi F1, 2 ; Dinarvand R1, 2
Authors

Source: Drug Research Published:2015


Abstract

The aim of the present work was to encapsulate 18-β-Glycyrrhetinic acid (GLA) in albumin conjugated poly(lactide-co-glycolide) (PLGA) nanoparticles by a modified nanoprecipitation method. Nanoparticles (NPs) were prepared by different drug to polymer ratios, human serum albumin (HSA) content, dithiothreitol (as producer of free thiol groups) content, and acetone (as non-solvent in nanoprecipitation). NPs with a size ranging from 126 to 174 nm were achieved. The highest entrapment efficiency (89.4±4.2%) was achieved when the ratio of drug to polymer was 1:4. The zeta potential of NPs was fairly negative (-8 to -12). Fourier transform infrared spectroscopy and differential scanning calorimetry proved the conjugation of HSA to PLGA NPs. In vitro release profile of NPs showed 2 phases: an initial burst for 4 h (34-49%) followed by a slow release pattern up to the end. The antibacterial effects of NPs against Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa were studied by microdilution method. The GLA-loaded NPs showed more antibacterial effect than pure GLA (2-4 times). The anticancer MTT test revealed that GLA-loaded NPs were approximately 9 times more effective than pure GLA in Hep G2 cells. © Georg Thieme Verlag KG Stuttgart, New York.
Other Related Docs
5. Albumin Nanostructures As Advanced Drug Delivery Systems, Expert Opinion on Drug Delivery (2016)
9. Recent Advances in Plga-Based Nanofibers As Anticancer Drug Delivery Systems, Journal of Drug Delivery Science and Technology (2023)
18. Anti-Cancer Drug Delivery Using Carbohydrate-Based Polymers, Current Pharmaceutical Design (2017)