Cetuximab Scfv-Modified 5-Fu Loaded Chitosan Nanoparticles: “A Novel Therapeutic Platform.”

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Cetuximab Scfv-Modified 5-Fu Loaded Chitosan Nanoparticles: “A Novel Therapeutic Platform.” Publisher Pubmed

Jalalvand M¹ ; Esmaeili F² ; Falahzadeh K¹ ; Mazloumi M¹ ; Bayat E⁴ ; Zandsalimi F⁵ ; Talebkhan Y⁴ ; Nematollahi L⁴

Source: Current Pharmaceutical Biotechnology Published:2025

Abstract

Background: Colorectal cancer [CRC] is among the most fatal types of cancer. An active targeting delivery system that specifically interacts with CRC cells could improve the therapy's outcomes. Herein, Cetuximab single-chain fragment variable antibody [scFv] fragments were conjugated to the surface of 5-FU encapsulated chitosan nanoparticles [CS NPs] to develop an effective therapeutic platform [scFv-CS/5-FU NPs]. Method: CS/5-FU NPs were synthesized using a special fluidic system. Encapsulation efficiency [EE], loading capacity [LC], and the drug release profile of the particles were determined. scFv fragments were produced recombinantly and tailored on the surface of CS/5-FU NPs. The physicochemical features of scFv-CS/5-FU NPs were also characterized. MTT and flow cytometry assay investigated the toxicity effect of scFv-CS/5-FU NPs on the HCT116 cell line. Results: CS/5-FU NPs had a homogenous spherical shape. They possessed sustainable drug-release behavior. The produced scFv-CS/5-FU NPs were also spherical. scFv-CS/5-FU NPs significantly decreased the viability of cancerous cells in a dose-dependent manner and induced apoptosis in 97.97% of targeted cells. Conclusion: scFv-CS/5-FU NPs showed remarkable anti-CRC activity. This novel targeting delivery system reduced the effective dose of 5-FU which is of vital importance to decrease the devastating side effects of chemotherapy. © 2024 Bentham Science Publishers.

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Style	Citing Format
MLA	Jalalvand M, et al.. "Cetuximab Scfv-Modified 5-Fu Loaded Chitosan Nanoparticles: “A Novel Therapeutic Platform.”." Current Pharmaceutical Biotechnology, vol. , no. , 2025, pp. -.
APA	Jalalvand M, Esmaeili F, Falahzadeh K, Mazloumi M, Bayat E, Zandsalimi F, Talebkhan Y, Nematollahi L (2025). Cetuximab Scfv-Modified 5-Fu Loaded Chitosan Nanoparticles: “A Novel Therapeutic Platform.”. Current Pharmaceutical Biotechnology, (), -.
Chicago	Jalalvand M, Esmaeili F, Falahzadeh K, Mazloumi M, Bayat E, Zandsalimi F, Talebkhan Y, Nematollahi L. "Cetuximab Scfv-Modified 5-Fu Loaded Chitosan Nanoparticles: “A Novel Therapeutic Platform.”." Current Pharmaceutical Biotechnology , no. (2025): -.
Harvard	Jalalvand M et al. (2025) 'Cetuximab Scfv-Modified 5-Fu Loaded Chitosan Nanoparticles: “A Novel Therapeutic Platform.”', Current Pharmaceutical Biotechnology, (), pp. -.
Vancouver	Jalalvand M, Esmaeili F, Falahzadeh K, Mazloumi M, Bayat E, Zandsalimi F, et al.. Cetuximab Scfv-Modified 5-Fu Loaded Chitosan Nanoparticles: “A Novel Therapeutic Platform.”. Current Pharmaceutical Biotechnology. 2025;():-.
BibTex	@article{ author = {Jalalvand M and Esmaeili F and Falahzadeh K and Mazloumi M and Bayat E and Zandsalimi F and Talebkhan Y and Nematollahi L}, title = {Cetuximab Scfv-Modified 5-Fu Loaded Chitosan Nanoparticles: “A Novel Therapeutic Platform.”}, journal = {Current Pharmaceutical Biotechnology}, volume = {}, number = {}, pages = {-}, year = {2025} }
RIS	TY - JOUR AU - Jalalvand M AU - Esmaeili F AU - Falahzadeh K AU - Mazloumi M AU - Bayat E AU - Zandsalimi F AU - Talebkhan Y AU - Nematollahi L TI - Cetuximab Scfv-Modified 5-Fu Loaded Chitosan Nanoparticles: “A Novel Therapeutic Platform.” JO - Current Pharmaceutical Biotechnology VL - IS - SP - EP - PY - 2025 ER -

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