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Synergistic Effects of Polyaniline and Pulsed Electromagnetic Field to Stem Cells Osteogenic Differentiation on Polyvinylidene Fluoride Scaffold Publisher Pubmed



Mirzaei A1, 2 ; Saburi E3 ; Enderami SE4, 5, 6 ; Barati Bagherabad M7 ; Enderami SE4, 5, 6 ; Chokami M9 ; Shapouri Moghadam A10 ; Salarinia R11 ; Ardeshirylajimi A12 ; Mansouri V13 ; Soleimanifar F9, 14
Authors

Source: Artificial Cells# Nanomedicine and Biotechnology Published:2019


Abstract

Repairing the lost or damaged mandible is very difficult and time-consuming, so there is a great hope for tissue engineering to accelerate it. At the present study, electrospinning was applied to fabricate polyvinylidene fluoride (PVDF) and PVDF-polyaniline (PANI) composite scaffolds. In addition, extremely low frequency pulsed electromagnetic field (PEMF) was applied for treating the stem cells derived from dental pulp (DPSCs) when cultured on the nanofibrous scaffolds. Osteoinductive property of the fabricated PVDF, PVDF-PANI scaffold at the presence and absence of the PEMF was investigated by evaluating the common osteogenic differentiation markers in seeded-DPSCs on the scaffold. Results demonstrated that cell attachment, protein adsorption and cells viability were increased when PEMF was applied. In addition, ALP activity, calcium content, osteogenic genes and protein evaluations confirmed that PEMF could significantly increase osteoinductivity of the PVDF while composite with PANI. According to the results, the use of polymers with piezoelectricity and conductivity features plus PEMF exposure has a promising potential to improve the current treatment methods in bone and mandibular defects. © 2019, © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
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